Enhanced Validation

Anti-ALDH1A3 Antibody

aldehyde dehydrogenase 1 family, member A3
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human ALDH1A3
Alternative Gene Names
ALDH6, RALDH3
Price
$445.00
Product Number
HPA046271
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
aldehyde dehydrogenase 1 family, member A3
Target Gene
ALDH1A3
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ERGGAMATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFIN
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000052070 (83%)

Mouse ENSMUSG00000015134 (83%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
aldehyde dehydrogenase 1 family, member A3
Gene Name
ALDH1A3
Alternative Gene Names
ALDH6, RALDH3
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000184254 on the Human Protein Atlas

Shehata M, Kim H, Vellanki R, Waterhouse PD, Mahendralingam M, Casey AE, Koritzinsky M, Khokha R

Identifying the murine mammary cell target of metformin exposure

Commun Biol , 2019 May 20; 2:192. Epub 2019 May 20

PubMed ID: 31123716 DOI: 10.1038/s42003-019-0439-x

Giraddi RR, Shehata M, Gallardo M, Blasco MA, Simons BD, Stingl J

Stem and progenitor cell division kinetics during postnatal mouse mammary gland development

Nat Commun , 2015 Oct 29; 6:8487. Epub 2015 Oct 29

PubMed ID: 26511661 DOI: 10.1038/ncomms9487

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Alternative Antibodies
Corresponding Antigens

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