Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000026319 (86%)
Mouse ENSMUSG00000040407 (83%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells
Curr Biol , 2017 Oct 9; 27(19):2999-3009.e9
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