Enhanced Validation

Polyclonal Anti-AIF1 Antibody

allograft inflammatory factor 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human AIF1
Alternative Gene Names
AIF-1, Em:AF129756.17, IBA1, IRT-1
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
allograft inflammatory factor 1
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000000853 (84%)

Mouse ENSMUSG00000024397 (84%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
allograft inflammatory factor 1
Gene Name
Alternative Gene Names
AIF-1, Em:AF129756.17, IBA1, IRT-1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000204472 on the Human Protein Atlas

Cameron SA, White SM, Arrollo D, Shulman ST, Rowley AH

Arterial immune protein expression demonstrates the complexity of immune responses in Kawasaki disease arteritis

Clin Exp Immunol , 2017 Aug 7; 190(2):244-250. Epub 2017 Aug 7

PubMed ID: 28707750 DOI: 10.1111/cei.13010

Rowley AH, Baker SC, Kim KY, Shulman ST, Yang A, Arrollo D, DeBerge M, Han S, Sibinga NE, Pink AJ, Thorp EB

Allograft Inflammatory Factor-1 Links T-Cell Activation, Interferon Response, and Macrophage Activation in Chronic Kawasaki Disease Arteritis

J Pediatric Infect Dis Soc , 2017 May 15; 6(3):e94-e102. Epub 2017 May 15

PubMed ID: 28505365 DOI: 10.1093/jpids/pix025

Koeppen AH, Ramirez RL, Becker AB, Mazurkiewicz JE

Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation

Acta Neuropathol Commun , 2016 May 3; 4:46. Epub 2016 May 3

PubMed ID: 27142428 DOI: 10.1186/s40478-016-0288-5

Silva K, Hope-Lucas C, White T, Hairston TK, Rameau T, Brown A

Cortical neurons are a prominent source of the proinflammatory cytokine osteopontin in HIV-associated neurocognitive disorders

J Neurovirol , 2015 Apr; 21(2):174-185. Epub 2015 Jan 31

PubMed ID: 25636782 DOI: 10.1007/s13365-015-0317-3

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