Enhanced Validation

Polyclonal Anti-AHNAK2 Antibody

AHNAK nucleoprotein 2
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human AHNAK2
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
AHNAK nucleoprotein 2
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000072812 (67%)

Rat ENSRNOG00000028545 (63%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
AHNAK nucleoprotein 2
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000185567 on the Human Protein Atlas

Koguchi D, Matsumoto K, Shimizu Y, Kobayashi M, Hirano S, Ikeda M, Sato Y, Iwamura M

Prognostic Impact of AHNAK2 Expression in Patients Treated with Radical Cystectomy

Cancers (Basel) , 2021 Apr 9; 13(8):1748. Epub 2021 Apr 9

PubMed ID: 33918555 DOI: 10.3390/cancers13081748

Zhang S, Lu Y, Qi L, Wang H, Wang Z, Cai Z

AHNAK2 Is Associated with Poor Prognosis and Cell Migration in Lung Adenocarcinoma

Biomed Res Int , 2020 Aug 20; 2020:8571932. Epub 2020 Aug 20

PubMed ID: 32904605 DOI: 10.1155/2020/8571932

Lu D, Wang J, Shi X, Yue B, Hao J

AHNAK2 is a potential prognostic biomarker in patients with PDAC

Oncotarget , 2017 Mar 7; 8(19):31775-31784. Epub 2017 Mar 7

PubMed ID: 28423668 DOI: 10.18632/oncotarget.15990

Fagerberg L, Oksvold P, Skogs M, Algenäs C, Lundberg E, Pontén F, Sivertsson A, Odeberg J, Klevebring D, Kampf C, Asplund A, Sjöstedt E, Al-Khalili Szigyarto C, Edqvist PH, Olsson I, Rydberg U, Hudson P, Ottosson Takanen J, Berling H, Björling L, Tegel H, Rockberg J, Nilsson P, Navani S, Jirström K, Mulder J, Schwenk JM, Zwahlen M, Hober S, Forsberg M, von Feilitzen K, Uhlén M

Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).

J Proteome Res , 2013 Jun 7; 12(6):2439-48. Epub 2012 Dec 31

PubMed ID: 23276153 DOI: 10.1021/pr300924j

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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