Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000018655 (92%)
Mouse ENSMUSG00000022407 (92%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer
Nat Commun , 2019 Nov 15; 10:5177. Epub 2019 Nov 15
Metabolomic profiling of human lung tumor tissues – nucleotide metabolism as a candidate for therapeutic interventions and biomarkers
Mol Oncol , 2018 Sep 13; 12(10):1778-1796. Epub 2018 Sep 13
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency.
Hum Mol Genet , 2012 Apr 1; 21(7):1534-43. Epub 2011 Dec 16
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