Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000054808 (99%)
Rat ENSRNOG00000020433 (98%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Nanoscale Imaging of Kidney Glomeruli Using Expansion Pathology
Front Med (Lausanne) , 2018 Nov 21; 5:322. Epub 2018 Nov 21
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
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