Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000016265 (77%)
Mouse ENSMUSG00000024981 (76%)
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Lipid sensing by mTOR complexes via de novo synthesis of phosphatidic acid
J Biol Chem , 2017 Feb 21; 292(15):6303-6311. Epub 2017 Feb 21
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