Enhanced Validation

Anti-ACO2 Antibody

aconitase 2, mitochondrial
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human ACO2
Alternative Gene Names
ACONM
Price
$445.00
Product Number
HPA001097
Unit Size
Concentration
Lot dependent
Availability
7 in Stock
Ships
Ready to Ship
Target Protein
aconitase 2, mitochondrial
Target Gene
ACO2
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEG
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022477 (97%)

Rat ENSRNOG00000024128 (97%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
aconitase 2, mitochondrial
Gene Name
ACO2
Alternative Gene Names
ACONM
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000100412 on the Human Protein Atlas

Stumpf SK, Berghoff SA, Trevisiol A, Spieth L, Düking T, Schneider LV, Schlaphoff L, Dreha-Kulaczewski S, Bley A, Burfeind D, Kusch K, Mitkovski M, Ruhwedel T, Guder P, Röhse H, Denecke J, Gärtner J, Möbius W, Nave KA, Saher G

Ketogenic diet ameliorates axonal defects and promotes myelination in Pelizaeus–Merzbacher disease

Acta Neuropathol , 2019 Mar 27; 138(1):147-161. Epub 2019 Mar 27

PubMed ID: 30919030 DOI: 10.1007/s00401-019-01985-2

Latonen L, Afyounian E, Jylhä A, Nättinen J, Aapola U, Annala M, Kivinummi KK, Tammela TT, Beuerman RW, Uusitalo H, Nykter M, Visakorpi T

Integrative proteomics in prostate cancer uncovers robustness against genomic and transcriptomic aberrations during disease progression

Nat Commun , 2018 Mar 21; 9:1176. Epub 2018 Mar 21

PubMed ID: 29563510 DOI: 10.1038/s41467-018-03573-6

Ilgen P, Stoldt S, Conradi LC, Wurm CA, Rüschoff J, Ghadimi BM, Liersch T, Jakobs S

STED Super-Resolution Microscopy of Clinical Paraffin-Embedded Human Rectal Cancer Tissue

PLoS One , 2014; 9(7):e101563. Epub 2014 Jul 15

PubMed ID: 25025184 DOI: 10.1371/journal.pone.0101563

Cantu D, Fulton RE, Drechsel DA, Patel M

Mitochondrial aconitase knockdown attenuates paraquat-induced dopaminergic cell death via decreased cellular metabolism and release of iron and H₂O₂.

J Neurochem , 2011 Jul; 118(1):79-92. Epub 2011 May 19

PubMed ID: 21517855 DOI: 10.1111/j.1471-4159.2011.07290.x

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Corresponding Antigens

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