Polyclonal Anti-ACLY Antibody
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Genetic validation in WB by siRNA knockdown.
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000016924 (98%)
Mouse ENSMUSG00000020917 (98%)
Recommended conditions:
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibodies Anti-ACLY HPA022959, Anti-ACLY HPA022434, Anti-ACLY HPA028758.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000131473 on the Human Protein Atlas
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