Enhanced Validation

Polyclonal Anti-A2ML1 Antibody

alpha-2-macroglobulin-like 1
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human A2ML1
Alternative Gene Names
CPAMD9, FLJ25179
Open Datasheet
Price
$505.00
Product Number
HPA038848
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[6 pictures]
Immunohistochemistry analysis in human esophagus and liver tissues using HPA038848 antibody. Corresponding A2ML1 RNA-seq data are presented for the same tissues.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
alpha-2-macroglobulin-like 1
Target Gene
A2ML1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
RYTYGKPMLGAVQVSVCQKANTYWYREVEREQLPDKCRNLSGQTDKTGCFSAPVDMATFDLIGYAYSHQINIVATVVEEGTGVEANATQNIY
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000045772 (37%)

Mouse ENSMUSG00000030111 (36%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-A2ML1 HPA038847.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA038848 on the Human Protein Atlas
Protein Name
alpha-2-macroglobulin-like 1
Gene Name
A2ML1
Alternative Gene Names
CPAMD9, FLJ25179
UniProt ID
A8K2U0
Gene (Ensembl)
ENSG00000166535
Entrez Gene ID
Entrez gene: 144568
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000166535 on the Human Protein Atlas

Su L, Zhang G, Kong X

Prognostic Significance of Pregnancy Zone Protein and Its Correlation with Immune Infiltrates in Hepatocellular Carcinoma

Cancer Manag Res , 2020 Oct 9; 12:9883-9891. Epub 2020 Oct 9

PubMed ID: 33116846 DOI: 10.2147/CMAR.S269215

Kashiwagi H, Ishimoto H, Izumi SI, Seki T, Kinami R, Otomo A, Takahashi K, Kametani F, Hirayama N, Sasaki E, Shiina T, Sakabe K, Mikami M, Kametani Y

Human PZP and common marmoset A2ML1 as pregnancy related proteins

Sci Rep , 2020 Mar 20; 10:5088. Epub 2020 Mar 20

PubMed ID: 32198464 DOI: 10.1038/s41598-020-61714-8

Månberg A, Bradley F, Qundos U, Guthrie BL, Birse K, Noël-Romas L, Lindskog C, Bosire R, Kiarie J, Farquhar C, Burgener AD, Nilsson P, Broliden K

A High-throughput Bead-based Affinity Assay Enables Analysis of Genital Protein Signatures in Women At Risk of HIV Infection

Mol Cell Proteomics , 2019 Mar; 18(3):461-476

PubMed ID: 30504243 DOI: 10.1074/mcp.RA118.000757

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-A2ML1 (HPA038847)
Corresponding Antigens
PrEST Antigen A2ML1 (APrEST81147)

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