Enhanced Validation

Monoclonal Anti-WWTR1 Antibody

WW domain containing transcription regulator 1
References (3)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human WWTR1
Alternative Gene Names
DKFZp586I1419, TAZ
Open Datasheet
Price
$505.00
Product Number
AMAb90730
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human placenta and lymph node tissues using AMAb90730 antibody. Corresponding WWTR1 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-WWTR1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[1 pictures]
Immunofluorescence staining in SKMEL cell line with Anti-WWTR1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
WW domain containing transcription regulator 1
Target Gene
WWTR1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000027803 (96%)

Rat ENSRNOG00000016617 (96%)

Clonality
Monoclonal
Clone ID
CL0371
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90730 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90730 on the Human Protein Atlas
Protein Name
WW domain containing transcription regulator 1
Gene Name
WWTR1
Alternative Gene Names
DKFZp586I1419, TAZ
UniProt ID
Q9GZV5
Gene (Ensembl)
ENSG00000018408
Entrez Gene ID
Entrez gene: 25937
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Cigliano A, Zhang S, Ribback S, Steinmann S, Sini M, Ament CE, Utpatel K, Song X, Wang J, Pilo MG, Berger F, Wang H, Tao J, Li X, Pes GM, Mancarella S, Giannelli G, Dombrowski F, Evert M, Calvisi DF, Chen X, Evert K

The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease

J Exp Clin Cancer Res , 2022 Jun 3; 41:192. Epub 2022 Jun 3

PubMed ID: 35655220 DOI: 10.1186/s13046-022-02394-2

Zhang S, Zhang J, Evert K, Li X, Liu P, Kiss A, Schaff Z, Ament C, Zhang Y, Serra M, Evert M, Chen N, Xu F, Chen X, Tao J, Calvisi DF, Cigliano A

The Hippo Effector Transcriptional Coactivator with PDZ-Binding Motif Cooperates with Oncogenic β-Catenin to Induce Hepatoblastoma Development in Mice and Humans

Am J Pathol , 2020 Jul; 190(7):1397-1413

PubMed ID: 32283103 DOI: 10.1016/j.ajpath.2020.03.011

García P, Rosa L, Vargas S, Weber H, Espinoza JA, Suárez F, Romero-Calvo I, Elgueta N, Rivera V, Nervi B, Obreque J, Leal P, Viñuela E, Aguayo G, Muñiz S, Sagredo A, Roa JC, Bizama C

Hippo-YAP1 Is a Prognosis Marker and Potentially Targetable Pathway in Advanced Gallbladder Cancer

Cancers (Basel) , 2020 Mar 25; 12(4):778. Epub 2020 Mar 25

PubMed ID: 32218280 DOI: 10.3390/cancers12040778

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Alternative Antibodies
Anti-WWTR1 (AMAb90729)
Anti-WWTR1 (HPA007415)
Corresponding Antigens
PrEST Antigen WWTR1 (APrEST70841)

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