Triple A Polyclonals
Triple A Polyclonals offer specificity, reproducibility and versatility
Triple A Polyclonals™ (Atlas Antibodies Advanced Polyclonals) are research-grade rabbit polyclonals developed within the Human Protein Atlas project. Triple A Polyclonals are specific, reproducible and versatile. Read below to learn about their unique advantages.
Unique Features of Triple A Polyclonals
- 21,000 rabbit polyclonal antibodies
- Covers 75% of the human proteome
- Validated for IHC, WB, ICC-IF
- 500 IHC images for each antibody on the Human Protein Atlas.
- Several epitopes for high sensitivity.
- Unique antigen design for optimal specificity.
- Ensured lot-to-lot reproducibility.
- Enhanced Validation as an added layer of security.
Triple A Polyclonals on the Human Protein Atlas
The Triple A Polyclonals have been used to map the human proteome by the Human Protein Atlas project. Each antibody has been used for IHC-staining of samples from 708 individuals originating from normal and cancer afflicted human tissues. Trained pathologists have evaluated the reliability of all IHC-staining patterns. In addition, ICC-IF-based confocal microscopy has been used to study subcellular distribution patterns in selected cell lines. Further, Triple A Polyclonals are characterized by Western Blot (WB), primarily in endogenous protein lysates from human tissues.
Triple A Polyclonals are carefully designed using a proprietary antigen design software that selects a Protein Epitope Signature Tag (PrEST) sequence of between 50 to 150 amino acids having the lowest possible identity to other human proteins.
The PrEST concept secures the highest level of specificity when used for polyclonal antibody production. While full-length recombinant proteins often increase the risk for off-target binding, the PrEST antigen effectively reduces unwanted specificity by avoiding regions with high regional or local sequence identity to other proteins.
Developed by top Swedish scientists to create the Human Protein Atlas, our carefully designed Triple A Polyclonals are produced using the same standardized production process to ensure consistency. Furthermore, our unique antibody purification process, using the recombinant PrEST-antigen as the affinity ligand, achieves the highest reproducibility level. New production lots are tested in parallel with existing lots to ensure functionality and assay reproducibility in a real lab situation.
Triple A Polyclonals combine optimal specificity with superior versatility across applications, including IHC, ICC-IF, and WB. While anti-peptide antibodies often generate too narrowly specific antibodies, the use of our PrEST-antigens results in advanced polyclonals that combine optimal specificity and minimal off-target binding with superior epitope recognition across applications, protein states and species. If you would like to use Triple A Polyclonals in an application or species we have not yet evaluated, we encourage you to participate in our Explorer Program.
All products from Atlas Antibodies are designed for the highest possible performance and are manufactured using a standardized process to ensure the most rigorous levels of quality. Our guarantee is valid one year from the date of purchase.
Are you using our antibodies in an application or with a species we don't show on our product pages? Then we hope you will join our Explorer Program. We offer a 50% refund or a 50% discount on your next vial if you share your results with us.
Antibody Co-Development Program
Can’t find an antibody for the protein you are working on in our catalog? Join our co-development program and let us develop it together. We are always interested in receiving your request.
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