Neuroscience Markers
The neuroscience marker antibody panel consists of PrecisA Monoclonals™ designed to specifically recognize the main anatomical and neurochemical cell types in rodent and human nervous system.
The neuroscience marker panel
The antibodies in the neuroscience marker panel are tools for mapping the structures and cell types in the central and peripheral nervous system. The neuroscience marker panel consists of 34 antibodies targeting neural lineage markers and signaling markers. The panel includes neural lineage markers for neurons, astrocytes and oligodendrocytes/Schwann cells. Signaling markers target glutamate, GABA, acetylcholine, noradrenaline, dopamine and serotonin systems.
- Selected target proteins are expressed only by a single cell type.
- IHC-validation in rat, mouse, and human tissues.
- WB-validation in mouse and human tissue lysates for the majority of the markers.
- Antibodies of different isotypes, allowing for multiplexing experiments.
Explore Neuroscience Markers
The complete neuroscience marker antibody panel is presented in our product catalog.
Markers for neural lineage and signaling
The image below shows a coronal section of rat brain labeled with markers for three different cell types, including neurons, oligodendrocytes, and astrocytes. The antibodies can all be found in the Neuroscience Marker panel: Anti-NEFM (AMAb91030), Anti-CNP (AMAb91068) and Anti-GFAP (AMAb91033) respectively.
Below, some of the major brain neurotransmitter systems are shown on a sagittal mouse brain section. The image demonstrates the GABAergic system, glutamatergic system, and acetylcholine system, here visualized by the Anti-GAD1 (AMAb91076), Anti-VGLUT1(AMAb91041) and Anti-CHAT (AMAb91129) antibodies respectively.
All PrecisA Monoclonals are isotyped. Multiple antibodies of different isotypes targeting the same marker allow you to choose the antibodies that best suit your experiment, as well as allow for multiplexed experiments using antibodies of different isotypes as exemplified above.
Anti-NET, an example of a neurochemical cell type marker
One of the neuroscience marker antibodies we offer is the Anti-NET (AMAb91116) monoclonal antibody. This antibody recognizes the norepinephrine/noradrenaline transporter (NET, SLC6A2) and can be used to detect both noradrenergic cell bodies and processes in rat, mouse and human nervous system. The Anti-NET antibody is highly specific and does not show any cross-reactivity with dopamine transporter (SLC6A3, DAT).
High specificity and interspecies reactivity
The images below show specific staining of noradrenergic cell bodies and fibers in rat locus coeruleus (A), noradrenergic fibers in mouse cerebral cortex (B) and noradrenergic cell bodies and fibers in human locus coeruleus (C). The specificity and selectivity of the Anti-NET antibody are further demonstrated on images D and E. These images show a coronal section of rat brain at the level of caudate-putamen stained with Anti-NET in green and Anti-DAT (AMAb91125) in magenta. Note the specific staining with both antibodies: the thicker, varicose noradrenaline fibers are mainly observed in the cortex medial septum and diagonal band of Broca area, while a dense network of thin dopamine fibers is seen in caudate-putamen (E). The caudate-putamen is virtually devoid of noradrenaline fibers, only single ones can sometimes be observed, e.g. in the dorsal part of caudate-putamen (D), high power of the boxed area in E.
