Laminin Markers

The Laminin Markers are PrecisA Monoclonals™, mouse monoclonal antibodies targeting different laminin subunits. The PrecisA laminin antibodies are tools to identify specific laminin subunits present in a tissue of interest to further understand the roles and expression patterns of different laminins.

Laminin proteins are the major components of the basement membrane. They are crucial for a wide variety of biological processes, such as cell adhesion, migration, embryonic development, differentiation, and phenotypic stability. Laminins are a family of conserved multidomain, trimeric proteins that are part of the extracellular matrix structure. They are composed of three different subunits; an α-chain, a β-chain and a γ-chain, that can be found in five (α1-5), three (β1-3) and three (γ1-3) genetic variants respectively.

About BioLamina

About BioLamina

BioLamina develops, manufactures and distributes full length, human, recombinant laminins intended for biologically relevant cell culture. 

For more information about human recombinant laminin proteins, please visit BioLamina's science room.

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The Laminin Marker Panel

The Laminin Markers were developed in collaboration with the Swedish biotech company BioLamina AB. With the use of BioLamina’s human recombinant laminin isoforms, PrecisA Monoclonals selectively recognize the specific subunits of the laminins.

ELISA, Western Blot and Immunohistochemistry validations have been performed in order to select for specificity and rule out any cross-reactivity. The selection already started at the choice of the PrEST Antigens. The criteria for our laminin antibodies is that they must be specific for each subunit without any cross-reactivity. The different specific laminin subunit antibodies present a tool for researchers that will aid in clarifying which laminins are present in the tissue of interest. The monoclonal antibodies have been developed using the same stringent conditions as all PrecisA Monoclonal antibodies, which guarantees a secured continuity and stable supply.

Explore Laminin Markers

Explore Laminin Markers

The complete laminin marker antibody panel is presented in our product catalog.

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Combine different laminin antibodies

Combine the different laminin antibodies to target specific laminins. For example, laminin-421 consists of alpha 4, beta 2 and gamma 1 and will be detected by the Anti-LAMA4, Anti-LAMB2 and Anti-LAMC1 antibodies from the marker panel.

LAMC1, an example of the development of specific laminin antibodies.

The laminin antibodies are carefully selected to recognize only one specific laminin subunit and the antibodies, therefore, they are used as a tool to detect which specific laminins are expressed in the tissue of interest. The antibody selection process starts already during the antigen selection phase and continued throughout the development and QC processes.

ELISA based functional characterization screen

Here we show how a monoclonal antibody targeting laminin gamma 1 (Anti-LAMC1) has been developed and selected through our process and can be used to detect Laminin gamma 1 expression in tissue.

ELISA of Anti-LAMC1 Antibody (AMAb91138)
ELISA of Anti-LAMC1 Antibody (AMAb91138) shows specific binding to human recombinant laminins containing gamma 1 subunit while no binding to Laminin-332, containing gamma 2 subunit

All our laminin antibodies have gone through an ELISA-based functional characterization screening to select and confirm that they are specific for only the corresponding laminin subunit and do not cross-react with other subunits. This has been done using ELISA-plates coated with different human recombinant laminin isoforms.

Subunit specificity confirmed by Western Blot

For further characterization, we have confirmed subunit specificity in WB using the same human recombinant laminins as for ELISA. Data below shows that the antibody only detects its specific subunit and does not display any cross-reactivity.

Western blot of Anti-LAMC1 (AMAb91138)
Western blot loaded with expressed human laminins as indicated, showing that Anti-LAMC1 (AMAb91138) is only recognizing laminins containing laminin gamma 1 (LAMC1) subunit.

Immunohistochemistry to confirm protein expression in relevant tissues

Finally, the antibodies were tested by IHC (see below) to confirm protein expression in relevant tissues and absence of non-specific staining.

Taken together data confirms that the Anti-LAMC1 antibody is highly specific and selective for its target subunit in various applications.

Anti-LAMC1 (AMAb91138) used foIHC staining of human kidney
From the left: Anti-LAMC1 (AMAb91138) used for IHC staining of human kidney shows distinct staining of basement membranes in renal tubules and glomerulus. Staining of human colon shows strong positivity in basement membrane of glandular epithelium. Immunostaining of human endometrium demonstrates basement membrane of glandular epithelium. Basement membrane of squamous epithelium in human cervix is immunopositive for LAMC1.

Laminin-521, an example of how laminin antibodies can be used for detection and recognition of specific laminin subunits.

Laminin-521 is a protein naturally expressed by human pluripotent stem cells and is a critical factor of the pluripotent stem cell niche (Rodin et al, 2014Laperle, 2015). Laminin-521 is also crucially important for the development and function of several tissues, including heart muscle (Roediger, 2010) and retinal epithelium (Aisenbrey et al., 2006). In the adult kidney, laminin-521 is a key component of the glomerular basement membrane (Miner 2012).

The data below demonstrates how monoclonal antibodies against the laminin alpha 5, the beta 2 and the gamma 1 subunits can be used to detect the presence of laminin-521 in the kidney. The monoclonal antibodies used for this staining belong to different IgG isotypes, including IgG1, IgG2a, and IgG2b, which makes it possible to use them for multiplex IHC on the same tissue section.

An overlay image visualizing the three laminin subunits, including LAMA5, LAMB2 and LAMC1 in human kidney.
From the left: An overlay image visualizing the three laminin subunits, including LAMA5, LAMB2 and LAMC1 in human kidney. Arrows indicate the laminin-521 expression in the glomerular basement membrane. The individual images show basement membranes staining using Anti-LAMA5 (AMAb91124) in blue, Anti-LAMB2 (AMAb91097) in green and Anti-LAMC1 (AMAb91138) in red. Note that all three subunits are present in the basement membrane of glomerulus, while renal tubules express LAMA5 and LAMC1, but not LAMB2.