Cell Cycle Markers

The cell cycle is a series of processes, including cell proliferation, that a cell undergoes during its life cycle. Cancer lesions commonly originate from aberrant cellular proliferation.

Abnormal cellular proliferation can be caused by mutations or amplifications in the open-reading frames or regulatory domains of oncogenes and tumor suppressors that regulate cell growth.

Cell cycle regulation

Cell cycle progression is positively regulated and enforced by a family of serine/threonine protein kinases collectively referred to as cyclin-dependent kinases (CDKs). CDKs are activated by binding to respective cyclin partners and by phosphorylation.

These checkpoint pathways recognize and sense whether or not to initiate and/or promote cellular proliferation under a particular set of external and internal conditions. Crucial components of these pathways are mainly proteins encoded by some checkpoint genes including the tumor suppressor protein p53.

IHC staining of human squamous cell carcinoma using Anti-p53 Antibody (AMAb90946) — IHC staining of human squamous cell carcinoma using Anti-p53 Antibody (AMAb90946) showing strong nuclear positivity in tumor cells.

Mechanisms in Cancer

Mechanisms in Cancer

Download our white paper to learn about the mechanisms in cancer and protein markers used in cancer research.

Download white paper

Antibodies targeting cell cycle markers

Atlas Antibodies offer a range of primary antibodies targeting cell cycle markers. Selected antibodies are listed below.

IHC staining, WB analysis and ICC-IF staining using Anti-p53 antibody (AMAb90956). — Left: IHC staining of human colorectal cancer using the Anti-p53 antibody (AMAb90956) showing strong nuclear positivity in tumor cells but not in normal mucosa. Middle: WB analysis in U-251 cells transfected with control siRNA, target-specific siRNA probes using the same Anti-p53 antibody. Right: ICC-IF staining in A431 cells using the same Anti-p53 antibody, showing cell cycle-dependent nuclear (without nucleoli) staining in green. Microtubules are visualized in red.

Anti-p53 monoclonal antibody (AMAb90956)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 91%/75%

Anti-p53 polyclonal antibody (HPA051244)

validated for WB, ICC-IF
sequence identity mouse/rat 91%/75%

Anti-AURKA polyclonal antibody (HPA002636)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 66%/67%

Anti-AURKB polyclonal antibody (HPA037708)

validated for ICC-IF
sequence identity mouse/rat 55%/53%

Anti-CCNA1/CyclinA1 polyclonal antibody (HPA060646)

validated for ICC-IF
sequence identity mouse/rat 54%/53%

Anti-CCNB1/CyclinB1 polyclonal antibody (HPA030741)

validated for WB, ICC-IF
sequence identity mouse/rat 80%/78%

Anti-CCNB1/CyclinB1 polyclonal antibody (HPA061448)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 72%/68%

Anti-CCNB2/CyclinB2 Polyclonal antibody (HPA008873)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 81%/27%

Anti-CCNB3/CyclinB3 polyclonal antibody (HPA000496)

validated for IHC, ICC-IF
sequence identity mouse/rat 32%/29%

Anti-CCND1/ CyclinD1 polyclonal antibody (HPA027802)

validated for ICC-IF
sequence identity mouse/rat 89%/89%

Anti-CCND2/CyclinD2 polyclonal antibody (HPA049138)

validated for ICC-IF
sequence identity mouse/rat 74%/70%

Anti-CCND2/CyclinD2 polyclonal antibody (HPA054196)

validated for ICC-IF
sequence identity mouse/rat 94%/94%

Anti-CDK2 monoclonal antibody (AMAb91497)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 100%/100%

Anti-CDK4 monoclonal antibody (AMAb91499)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 94%/94%

Anti-CDK5 polyclonal antibody (HPA064535)

validated for IHC, ICC-IF
sequence identity mouse/rat 99%/99%

Anti-CDK6 polyclonal antibody (HPA002637)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 92%/92%

Anti-CDKN2A polyclonal antibody (HPA047838)

validated for ICC-IF
sequence identity mouse/rat 44%/43%

Anti-CDKN2B polyclonal antibody (HPA063327)

validated for IHC
sequence identity mouse/rat 62%/59%

Anti-CDKN2D polyclonal antibody (HPA043546)

validated for ICC-IF
sequence identity mouse/rat 79%/45%

Anti-CHEK1 polyclonal antibody (HPA044364)

validated for ICC-IF
sequence identity mouse/rat 93%/92%

Anti-CHEK2 polyclonal antibody (HPA001878)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 86%/86%

Anti-CHEK2 monoclonal antibody (AMAb91570)

validated for IHC, WB, ICC-IF
sequence identity mouse/rat 86%/86%

Anti-E2F1 polyclonal antibody (HPA008003)

validated for IHC
sequence identity mouse/rat 76%/76%

Anti-E2F1 polyclonal antibody (HPA029735)

validated for WB, ICC-IF
sequence identity mouse/rat 94%/93%

Anti-PLK1 polyclonal antibody (HPA051638)

validated for IHC
sequence identity mouse/rat 90%/88%

Anti-PLK1 polyclonal antibody (HPA053229)

validated for IHC
sequence identity mouse/rat 97%/85%

Anti-PLK1 monoclonal antibody (AMAb91515)

validated for WB
sequence identity mouse/rat 83%/91%

Anti-RB1 polyclonal antibody (HPA050082)

validated for IHC
sequence identity mouse/rat 89%/97%

Anti-RIF1 polyclonal antibody (HPA036887)

validated for IHC, ICC-IF
sequence identity mouse/rat 38%/36%

Anti-RIF1 polyclonal antibody (HPA036888)

validated for IHC
sequence identity mouse/rat 52%/49%