Loading Controls Panel
In addition to elucidating functional characteristics of proteins, subcellular location information can also facilitate protein interaction studies. For two proteins to interact, it is a prerequisite that they be located at the same defined site. Thus, knowing the subcellular location of a protein is a key step towards understanding function and probable interaction patterns.
PrecisA Monoclonals Loading Controls
The Loading Control Panel comprises PrecisA Monoclonals mouse antibodies designed to target specific proteins constitutively expressed across a diverse range of cell lines and tissues.
These antibodies undergo thorough characterization in four human cell lines (HeLa, HEK-293, A-431, Hep-G2) and two rodent cell lines (NIH-3T3 (mouse), NBT-II (rat)). The targets covered by this panel have molecular weights ranging from approximately 15 to 100 kDa.
A distinctive aspect of these antibodies is their development using a unique antigen design, accompanied by epitope mapping to precisely define specificity. To further ensure reliability, all panel members undergo genetic validation for Western blotting (WB) using the siRNA knockdown technique, confirming target specificity.
Multiplexing Capabilities
The Loading Control Panel seamlessly integrates with fluorescence-based multiplex Western blotting, offering a time-saving alternative to chemiluminescent detection.
For optimal multiplexing, select a loading control antibody with a different isotype than the antibody targeting the protein of interest (especially relevant when using mouse monoclonals). Alternatively, combine any panel member with one of our rabbit Triple A PolyclonalsTM.
By employing secondary antibodies conjugated with fluorescent dyes, the Western blot protocol can be streamlined to only one primary and one secondary incubation step. This innovative approach allows simultaneous visualization of the loading control and the protein of interest bands in a single image.
The PrecisA Monoclonals Loading Controls provide a robust and efficient solution for Western blot applications, ensuring accurate and reliable assessment of protein loading across diverse experimental conditions.