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Immunofluorescence in Cell Lines

Subcellular localization studies are a key step in elucidating protein function and interaction. This paper introduces you to how to study subcellular localization with Triple A Polyclonals using Immunofluorescence analysis on cells.

The need for subcellular localization studies
One major rationale for investigating the subcellular location of a specific protein is that location is often tightly connected to function. For example, proteins located in the nucleus are frequently implicated in gene regulation, proteins in mitochondria with energy production, and Golgi-related proteins are often associated with protein modification and sorting.

In addition to elucidating functional characteristics of proteins, subcellular location information can also facilitate protein interaction studies. For two proteins to interact, it is a prerequisite that they be located at the same defined site. Thus, knowing the subcellular location of a protein is a key step towards understanding function and probable interaction patterns.

  

Anti-ROCK2 Antibody
Anti-ROCK2 Antibody

Anti-ROCK2 Antibody

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Anti-ATP6V1D Antibody
Anti-ATP6V1D Antibody

Anti-ATP6V1D Antibody

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Anti-PPIL2 Antibody
Anti-PPIL2 Antibody

Anti-PPIL2 Antibody

HPA035344
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Anti-PIEZO2 Antibody

Anti-PIEZO2 Antibody

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Anti-POLR3A Antibody
Anti-POLR3A Antibody

Anti-POLR3A Antibody

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Anti-ST5 Antibody
Anti-ST5 Antibody

Anti-ST5 Antibody

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Anti-IFT22 Antibody
Anti-IFT22 Antibody

Anti-IFT22 Antibody

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Anti-SH2B2 Antibody
Anti-SH2B2 Antibody

Anti-SH2B2 Antibody

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Anti-TSPAN11 Antibody
Anti-TSPAN11 Antibody

Anti-TSPAN11 Antibody

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Anti-LAYN Antibody
Anti-LAYN Antibody

Anti-LAYN Antibody

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Anti-SH3TC2 Antibody
Anti-SH3TC2 Antibody

Anti-SH3TC2 Antibody

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Anti-RPS27A Antibody

Anti-RPS27A Antibody

HPA054087
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The Subcellular Atlas on Human Protein Atlas 

Subcellular location studies are a key step in elucidating protein function and interaction. ICC-IF images showing the localizations for all human protein targets are accessible through the Human Protein Atlas portal.

On the Human Protein Atlas, subcellular location information of proteins is obtained by using high-resolution fluorescent confocal microscopy in three different human cell lines. Each protein is assigned to one or several of the 30 subcellular locations by manual annotation.

All ICC-IF images shown on the Human Protein Atlas portal can be visualized through the interactive “virtual microscope”, enabling the user to toggle the different clickable multicolor images, which display the staining for the protein of interest as well as the organelle probes.

Explore the Subcellular Atlas

Download the "Immunofluorescence in Cell Lines" white paper and learn about:

  • The need for subcellular localization studies
  • The Human Protein Atlas
  • ICC-IF on the Human Protein Atlas

Immunofluorescence in Cell Lines