Research Roundup: Our Antibodies for Neuroscience in Action

Our primary antibodies are used in cutting-edge neuroscience research. All our neuroscience products are highly validated and tailor-made for research on signaling, neural lineage, developmental processes, aging, and neurodegenerative disorders.

Our primary antibodies are created using a unique antigen design for optimal specificity, affinity-purified using the immunogen, and manufactured using a standardized production process to ensure the highest quality. 

Let's take a look at two recent studies showing our products in action.

1. Sideroflexin 3 is a mitochondrial protein enriched in neurons 

The mitochondrial protein sideroflexin 3 (Sfxn3) is one of the main Sfxn proteins responsible for transporting the amino acid serine into mitochondria during one-carbon metabolism.  

Sfx3n is highly expressed in the brain and thought to be involved in Parkinson´s disease's neurodegenerative process.  

In this study, the authors took a detailed look into the spatiotemporal brain localization of Sfx3n in the rat. They found a sharp mitochondrial increase in Sfx3n in the rat brain after birth, suggesting that Sf3x3 is necessary for neurite outgrowth and synapse formation. 

This finding provides a translatable baseline for investigating presumed alterations in Sfx3n toward clarifying the pathogenesis of human neurological and neurodegenerative conditions. 

This study used our rabbit polyclonal anti-Sfxn3 antibody (HPA008028) in immunofluorescence and western blotting. 

REF: Rivell A, Petralia RS, Wang YX, Mattson MP, Yao PJ. Sideroflexin 3 is a mitochondrial protein enriched in neurons. Neuromolecular Med. 2019;21(3):314-321.

2. Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus

The hypothalamus is essential for homeostasis and energy metabolism. Developmental deficiencies of the hypothalamus can contribute to diseases or disorders of energy imbalance, obesity, and anxiety. However, many important aspects of hypothalamic development remain unaddressed, particularly the progenitors responsible for neurogenesis in the hypothalamus.

In this study, the authors used single-cell RNA sequencing to unravel distinct transcriptional regulation circuits governing the development of distinct cell nuclei within the hypothalamus.

The authors identified several novels and specific markers for hypothalamic precursor cells and increased knowledge of the cellular and molecular mechanisms that underpin the development of the non-laminated mammalian hypothalamus. Another important point relates to the strong developmental conservation of nuclear structures in the hypothalamus and marker gene conservation between mice and humans.

Overall the results show that TTYH1-, FAM107A-, or HMGA2-expressing cells residing in the VZ were positive for SOX2 and P-VIM. The FAM107A+SOX2+ cells exhibited radial fibers extending basally toward the pial surface, suggesting that these cells are hypothalamic radial glial and hypothalamic mantle zone radial glial cells.

This study used our polyclonal rabbit anti-human TTYH1 antibody (HPA023617) in immunofluorescence.

REF:  Zhou, X., Zhong, S., Peng, H. et al. Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus. Nat Commun 11, 4063 (2020).