Research roundup: neuroscience antibodies and protein standards

Our highly-validated antibodies and QPrEST protein standards are used in cutting-edge neuroscience research. Read a roundup of some newly published studies to see our neuroscience antibodies and targeted proteomics in action!

Our antibodies and QPrEST protein standards are used in cutting-edge neuroscience research. All our neuroscience products are highly validated and tailor-made for use in research on signaling, neural lineage, developmental processes, aging, and neurodegenerative disorders.

Our primary antibodies are created using a unique antigen design for optimal specificity, affinity-purified using the immunogen, and manufactured using a standardized production process to ensure the highest quality. 

Let's take a look at three recent studies showing our products in action.

1. Identification of novel cerebrospinal fluid biomarker candidates for dementia with Lewy bodies: a proteomic approach (van Steenoven et al. Mol Neurodegener, 2020)

Lewy body dementia (LBD) is associated with cognitive decline, parkinsonian symptoms, cognitive irregularities, and REM sleep behavior disorder.  

The diagnosis of LBD is often achievable upon post-mortem examination due to the overlap in symptoms with other disorders. This is why there is a need for new and accurate biomarkers to identify and categorize patients with the disease. 

In this work, the authors used mass spectrometry (to identifyand ELISA (to validatesix promising biomarkers in the cerebral spinal fluid of LBD patients. The results suggest these candidate biomarkers  promise to improve diagnostic accuracy in living patientsparticularly when used as a panel. 

  • The study used our QPrEST20926 (neurosecretory protein VGF) as an internal standard.

2. Sideroflexin 3 is a mitochondrial protein enriched in neurons (Rivell et al., Neuromolecular Med, 2019)

atlas antibodies sfx3n

Fig. 1 Immunofluorescent staining with anti-Sfxn3 in human cell line U-2 OS shows localization to mitochondria in greenMicrotubules are shown in red, nuclei in blue—image source: Atlas Antibodies.

The mitochondrial protein sideroflexin 3 (Sfxn3) is one of the main Sfxn proteins responsible for transporting the amino acid serine into mitochondria during one-carbon metabolism.  

Sfx3n is highly expressed in the brain and thought to be involved in Parkinson´s disease's neurodegenerative process.  

In this study, the authors took a detailed look into the spatiotemporal brain localization of Sfx3n in the rat. They found a sharp mitochondrial increase in Sfx3n in the rat brain after birth, suggesting that Sf3x3 is important for neurite outgrowth and synapse formation. 

This finding provides a translatable baseline for the investigation of presumed alterations in Sfx3n toward clarifying the pathogenesis of human neurological and neurodegenerative conditions. 

3. Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus (Zhou et al. Nat Commun, 2020; 11:4063)

anti ttyh1 atlas antibodies

Fig. 2 Immunohistochemical staining of human cerebellum with anti-TTYH1 antibody shows distinct positivity in cells in the granular layer, in brown. Image source: Atlas Antibodies.

The hypothalamus is essential for homeostasis and energy metabolism. Developmental deficiencies of the hypothalamus can contribute to diseases or disorders of energy imbalance, obesity, and anxiety.

In this study, the authors used single-cell RNA sequencing to unravel distinct transcriptional regulation circuits governing the development of distinct cell nuclei within the hypothalamus.

The authors identified several novels and specific markers for hypothalamic precursor cells and increased knowledge of the cellular and molecular mechanisms that underpin the development of the non-laminated mammalian hypothalamus. Another important point relates to the strong developmental conservation of nuclear structures in the hypothalamus and marker gene conservation between mouse and human.


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