Primary antibodies are effective tools to analyze living cell components at the molecular level and identify proteins that may be involved in or cause disease. Our antibodies are used worldwide!
For our Research Roundup series, we present you with three studies that have used our primary antibodies to better understand the role of kinesin’s motor domain in microtubules, membranes & trafficking in organelles, and lysosomal protein composition.
1. The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum
Kinesin is a molecular motor that moves along the microtubules carrying proteins up and down the microtubules. The kinesin family of microtubule motors is divided into subfamilies based on structure and function. For example, KIF9 is the founder of the Kinesin-9 subfamily.
In mice, KIF9 is testis-enriched, particularly in the mouse sperm flagellum. Deletion of the Kif9 gene results in male subfertility. However, it is still unclear if the motor domain of KIF9 is involved in normal sperm motility and male fertility.
To answer this question, this study used CRISPR/Cas9 system in mice to insert a mutation in a critical amino acid of the KIF9 motor domain, thus impairing kinesin’s motor activity. Specifically, the threonine of the ATP binding motif in the KIF9 motor domain was changed to asparagine (T100N).
The results show that T100N mutant mice exhibit reduced sperm motility and male fertility consistent with Kif9 knockout mice, clearly suggesting that the motor domain of KIF9 is essential for its localization in the sperm flagellum.
Since proteins of the axonemal cytoskeleton act as a scaffolding for various protein complexes and provide binding sites for molecular motor proteins such as kinesins, our rabbit polyclonal antibodies targeting axonemal proteins were used in western blot: anti‐DNAH17 (HPA024354), anti-GAS8 (HPA041311), and anti‐RSPH9 (HPA031703).
Myata et al., The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum. J-Stage, Experimental Animals, 2021