Two-years blog anniversary! A recap of our most popular posts
This month we celebrate the second birthday of our blog. If you have missed it read on. Here is a recap just for you.
How Enhanced Validation helps you publish your research
Do you know what to expect from a good antibody? How and when an antibody meets your expectations? Have you ever heard about enhanced validation? Read on to know more about it!
How to determine the specificity of an antibody for Western Blot
Paramount for a successful Western blot, is the ability of the primary antibody to detect the protein of interest. But how to validate an antibody for Western blot?
How to achieve greater separation in Western blot when proteins have a similar size
Despite its overall simplicity, the Western Blot application is a powerful procedure for the immunodetection of proteins and an essential tool within biological research. Nevertheless, proteins of the same length or similar size, such as isoform of the same protein, are difficult to separate. With some adjustments you can however achieve great results. Here we share some tips.
Happy one-year blog anniversary to us! Recap of our most popular posts
This month we celebrate the first anniversary of our blog. It has been an incredible journey. If you have missed our blog posts, read on. We have interviewed scientists, discussed the importance of antibody validation, the human protein atlas project and we gave you advice on how to succeed with your immunohistochemistry.
Contribute to advancing science by publishing your negative results
Have you ever obtained an amazing result that contradicts previous theory? If all looks well in the experiment, why not trust it?
How can I stain multiple proteins in the same tissue or cell?
Multiplexing-IHC simultaneously quantifies different immune markers on individual cells within different tumor types. But how do you stain multiple proteins in the same cell or tissue?
Can I use an antibody targeting a human protein in another model organism?
Can you use an antibody that targets a human protein in another model organism? Under the right conditions, the answer is yes!
Antigen retrieval - how to succeed with your IHC part II
How to unmask a protein in your sample? Read on to learn about the most common antigen retrieval methods used to restore epitope-antibody binding.
Tissue processing - how to succeed with your IHC
Tissue handling and tissue processing determine the quality of your IHC results. Do you want high resolution? Let’s understand how to achieve it.