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Antibodies Validated by the Human Protein Atlas

You focus on the research. We’ll handle the validation.

 

Developed and validated

  • Developed within the Human Protein Atlas
  • Validated with standardized protocols for IHC, WB and ICC-IF
  • 750+ antibodies independently tested for ChIP-exo-seq at Cornell University

Open data

  • Tissue expression profiles, staining images and validation results for every antibody freely available on proteinatlas.org
  • A widely used reference for protein expression in human tissues and cells
  • In-house control

Production and validation done in-house

  • 20 years of validation built on one principle: specificity shown, documented and open to inspection

 

The right antibody for your assay, from the start
 

BROWSE PRODUCTS

 

Advancing Antibody Validation:
Insights from Prof. Mathias Uhlén

Prof. Mathias Uhlén, Director of the Human Protein Atlas, presents the antibody validation proposal published in Nature Methods (2016). He explains why validation needs to be specific to the application and the context. He also describes how polyclonal antibodies support the Human Protein Atlas, through recognition of multiple epitopes and broad coverage of the human proteome.

 

 

 

How do we ensure antibody specificity and reproducibility?

Every antibody we sell is characterized and validated before release, using standardized protocols for each application. In this video, our scientists walk you through the process, from first characterization to batch-to-batch reproducibility testing in immunohistochemistry (IHC).

Setting the Standard: Antibodies You Can Trust

Application-Ready Results
Validated for IHC (44 human tissues), Western blot, immunofluorescence and ChIP.
✔ Performance you can expect, out of the box.

Consistent Performance, Batch After Batch
ISO 9001-certified production means every lot meets the same standard, so less re-validation.
✔ Same quality, every time.

Unrivaled Specificity
Unique PrEST antigens and enhanced validation give clean, reliable data with minimal off-target binding.
✔ See it in side-by-side image comparisons.

Total Transparency
Free access to all validation data, including high-resolution IHC images and protocols.
✔ Confidence through full visibility.

 

 

Enhanced Validation: Beyond Basic Validation

The Atlas Antibodies Difference

 

READ MORE

 

Get Reproducible Data. Faster.  

  • Antigen Design: Specificity from the Start

    Each antibody is developed against a recombinant human Protein Epitope Signature Tag (PrEST), typically 50–150 amino acids in length.
    These PrEST sequences are carefully selected to have minimal sequence similarity to other human proteins, significantly reducing the risk of cross-reactivity.

  • Functional Validation in Key Applications

    Our Triple A Polyclonals and PrecisA Monoclonals undergo functional validation in the most common antibody-based assays, including:

    • Immunohistochemistry (IHC)
    • Immunocytochemistry–Immunofluorescence (ICC–IF)
    • Western blot (WB)
    • Chromatin Immunoprecipitation ChIP

     

    For IHC, each antibody is evaluated in a broad panel of tissues, including 44 normal human tissues and the 20 most common cancer types. Performance is documented with over 500 high-resolution staining images per antibody, reviewed and annotated by expert researchers and pathologists. These images are available via the Human Protein Atlas.

  • Antibody Approval and Supporting Data

    Antibody specificity is further assessed through a combination of literature review, bioinformatics analysis, and comparison to RNA sequencing data to ensure biological relevance.

  • Commitment to Transparency

    All validation data, including images and experimental conditions, are freely available on the Atlas Antibodies and Human Protein Atlas websites.
    We also provide the exact immunogen sequence used for antibody generation, giving you complete insight into how each reagent was developed.

  • Validation vs. Reproducibility: Understanding the Difference

    While both validation and reproducibility are essential for antibody quality, they address distinct aspects of performance:

    Validation ensures that an antibody specifically binds to its intended target. Before any antibody is added to our catalog, it undergoes extensive validation. All validation data are documented and included on the antibody’s product data sheet.
    Reproducibility refers to the ability of an antibody to perform equally across different production lots. It ensures that a new batch behaves in the same way as the previous one, across all validated applications.

  • Our Approach to Reproducibility

    To guarantee reproducibility, each new antibody lot is tested against a reference lot. The comparison includes performance in all relevant applications (e.g., IHC, WB, ICC–IF), ensuring that the specificity and functionality remain consistent.

    This process allows us to maintain lot-to-lot consistency and provide researchers with reliable antibodies they can trust across long-term projects.

  • Example of reproducibility in IHC

    The standard test set-up for quality control of an antibody for the IHC application at Atlas Antibodies consists of a tissue microarray of more than 20 different samples of both normal and cancer tissues. The new lot of the antibody is tested and compared to the reference lot on consecutive sections from the tissue microarray.

    As the original manufacturer of all our products, we have full control of both the validation and reproducibility. You can trust that our antibodies are specific and perform consistently each time you use them.

    The image show examples from the quality control of antibody Anti-SATB2 (HPA001042) where a new lot is tested against its reference lot: IHC staining of human cerebral cortex, colon, liver and rectum using Anti-SATB2 antibody HPA001042 (A) shows similar protein distribution across tissues to independent Anti-SATB2 antibody HPA029543 (B).

Atlas Antibodies:

ISO certified according to ISO9001

 

Atlas Antibodies are ISO certified according to ISO9001 showing that our quality management system is up to that standard.

ISO 9001 is the internationally recognized standard for a quality management system (QMS) and organizations adhere to the standard to organize and continually improve business processes and efficiency.

This accreditation demonstrates Atlas Antibodies’ ongoing commitment to quality by satisfying customer requirements and industry specifications through an effective quality management system.