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Immunofluorescence in Cell Lines

Subcellular localization studies are a key step in elucidating protein function and interaction. This paper introduces you to how to study subcellular localization with Triple A Polyclonals using Immunofluorescence analysis on cells.

The need for subcellular localization studies
One major rationale for investigating the subcellular location of a specific protein is that location is often tightly connected to function. For example, proteins located in the nucleus are frequently implicated in gene regulation, proteins in mitochondria with energy production, and Golgi-related proteins are often associated with protein modification and sorting.

In addition to elucidating functional characteristics of proteins, subcellular location information can also facilitate protein interaction studies. For two proteins to interact, it is a prerequisite that they be located at the same defined site. Thus, knowing the subcellular location of a protein is a key step towards understanding function and probable interaction patterns.

  

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The Subcellular Atlas on Human Protein Atlas 

Subcellular location studies are a key step in elucidating protein function and interaction. ICC-IF images showing the localizations for all human protein targets are accessible through the Human Protein Atlas portal.

On the Human Protein Atlas, subcellular location information of proteins is obtained by using high-resolution fluorescent confocal microscopy in three different human cell lines. Each protein is assigned to one or several of the 30 subcellular locations by manual annotation.

All ICC-IF images shown on the Human Protein Atlas portal can be visualized through the interactive “virtual microscope”, enabling the user to toggle the different clickable multicolor images, which display the staining for the protein of interest as well as the organelle probes.

Explore the Subcellular Atlas

Download the "Immunofluorescence in Cell Lines" white paper and learn about:

  • The need for subcellular localization studies
  • The Human Protein Atlas
  • ICC-IF on the Human Protein Atlas

Immunofluorescence in Cell Lines