Our Anti-OTP for diagnostics and classification of lung cancer
Article: "Development and verification of new monoclonal orthopedia homeobox (OTP) specific antibodies for pulmonary carcinoid diagnostics"
Overview of the mAb isotyping and characterisation. (A) Linear representation of OTP protein (in grey) showing binding sites of mAb CL11222 and CL11225 (in purple), as well as pAb HPA039365 (in blue). (B) Representative images illustrating OTP IHC of both CL11222 and CL11225 in [1, 7] human hypothalamus, [2, 8] cerebral cortex, [3, 9] lung, [4, 10] kidney, [5, 11] small intestine, [6, 12] tonsil (magnification 200×). OTP, orthopedia homeobox; mAb, monoclonal antibody; pAb, polyclonal antibody; IHC, immunohistochemistry.
The results of our co-development project with the Department of Pathology, Maastricht University, using our Anti-OTP monoclonal antibodies on lung cancer tissue, have now been published in the Translational Lung Cancer Research journal!
Orthopedia homeobox (OTP) has shown to be a useful prognostic marker to predict the outcome of pulmonary carcinoids, which the World Health Organization also supports. However, the discontinuation of the initially used polyclonal antibody and the absence of a reliable, routinely applicable monoclonal OTP antibody hampers implementation in routine diagnostics.
Thanks to our co-development program, new monoclonal antibodies directed against OTP were developed and verified on formalin-fixed paraffin-embedded tissue of pulmonary neuroendocrine tumors (NETs) for clinical diagnostics.
We are confident that these novel monoclonal antibodies will improve routine diagnostics and current classification in the field of lung pathology.