Enhanced Validation

Anti-TMEM119 Antibody

transmembrane protein 119
Recommended Applications
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Product Description
Polyclonal Antibody against Human TMEM119
Price
$437.00
Product Number
HPA051870
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
transmembrane protein 119
Target gene
TMEM119
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVLEGAVVAGEGQGELEGSLLLAQEAQGPVGPPESPCACSSVHPS
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000054675 (52%)

Rat ENSRNOG00000000700 (46%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Protocols
Immunohistochemistry (IHC)

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-TMEM119 HPA052650.

Protein Name
transmembrane protein 119
Gene Name
TMEM119
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000183160 on the Human Protein Atlas

Zhang CJ, Jiang M, Zhou H, Liu W, Wang C, Kang Z, Han B, Zhang Q, Chen X, Xiao J, Fisher A, Kaiser WJ, Murayama MA, Iwakura Y, Gao J, Carman J, Dongre A, Dubyak G, Abbott DW, Shi FD, Ransohoff RM, Li X

TLR-stimulated IRAKM activates caspase-8 inflammasome in microglia and promotes neuroinflammation

J Clin Invest , 2018 Oct 29; 128(12):5399-5412. Epub 2018 Oct 29

PubMed ID: 30372424 DOI: 10.1172/JCI121901

Zheng P, Wang W, Ji M, Zhu Q, Feng Y, Zhou F, He Q

TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells

Oncol Lett , 2018 Mar 28; 15(6):8281-8286. Epub 2018 Mar 28

PubMed ID: 30112075 DOI: 10.3892/ol.2018.8358

Zrzavy T, Machado‐Santos J, Christine S, Baumgartner C, Weiner HL, Butovsky O, Lassmann H

Dominant role of microglial and macrophage innate immune responses in human ischemic infarcts

Brain Pathol , 2017 Dec 28; 28(6):791-805. Epub 2017 Dec 28

PubMed ID: 29222823 DOI: 10.1111/bpa.12583

Krasemann S, Madore C, Cialic R, Baufeld C, Calcagno N, El Fatimy R, Beckers L, O’Loughlin E, Xu Y, Fanek Z, Greco DJ, Smith ST, Tweet G, Humulock Z, Zrzavy T, Conde-Sanroman P, Gacias M, Weng Z, Chen H, Tjon E, Mazaheri F, Hartmann K, Madi A, Ulrich J, Glatzel M, Worthmann A, Heeren J, Budnik B, Lemere C, Ikezu T, Heppner FL, Litvak V, Holtzman DM, Lassmann H, Weiner HL, Ochando J, Haass C, Butovsky O

The TREM2-APOE pathway drives the transcriptional phenotype of dysfunctional microglia in neurodegenerative diseases

Immunity , 2017 Sep 19; 47(3):566-581.e9

PubMed ID: 28930663 DOI: 10.1016/j.immuni.2017.08.008

Thais F Galatro, Inge R Holtman, Antonio M Lerario, Ilia D Vainchtein, Nieske Brouwer, Paula R Sola, Mariana M Veras, Tulio F Pereira, Renata E P Leite, Thomas Möller, Paul D Wes, Mari C Sogayar, Jon D Laman, Wilfred den Dunnen, Carlos A Pasqualucci, Sueli M Oba-Shinjo, Erik W G M Boddeke, Suely K N Marie, Bart J L Eggen

Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

Nature Neuroscience , July 03, 2017

DOI: 10.1038/nn.4597

Zrzavy T, Hametner S, Wimmer I, Butovsky O, Weiner HL, Lassmann H

Loss of ‘homeostatic’ microglia and patterns of their activation in active multiple sclerosis

Brain , 2017 May 24; 140(7):1900-1913. Epub 2017 May 24

PubMed ID: 28541408 DOI: 10.1093/brain/awx113

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