Product Features
This Affibody® molecule binds to a protein of bacterial origin. It does not bind to human HER2 or EGFR. Cross reactivity with other species has not been tested.
The Affibody® Imaging Agent, Negative Control is modified with a unique C-terminal cysteine for directed single-point chemical modification, facilitating labeling to thiol reactive reagents, such as various fluorescent dyes.
Affibody® products are supplied for research use only and may not be used in a manufacturing process or in manufactured products or in human subjects. The Affibody® products shall in no event be used in medical or clinical applications, unless otherwise expressly stated by Affibody. The purchaser of Affibody® products is solely liable to ensure compliance with any regulatory requirements related to the purchaser’s use of Affibody® products.
Source: Recombinant protein produced in E. coli.
Specificity
Affibody® Imaging Agent, Negative Control binds to a protein of bacterial origin. It does not bind to human HER2 or EGFR. Cross reactivity with other species has not been tested.
Mw: 6.8 kDa
Theoretical pI: 4.6
Purity: >95 % as determined by RP-HPLC analysis.
Applications
E.g. small animal imaging.Conjugation: The Affibody® molecule contains a unique C-terminal cysteine ideal for directed chemical modifications. However, tail-to-tail dimers are spontaneously generated via a disulfide bridge between the C-terminal cysteines. Prior to coupling via the C-terminal cysteine, the Affibody® molecule needs to be reduced to expose the reactive cysteine residue. Recommended reducing condition is 20 mM DTT at a pH above 7.5 and incubation at room temperature for 2 hours. Remove excess DTT by passage through a desalting column, not by dialysis.
Form: Lyophilized protein. Lyophilized from 10 mM NH₄HCO₃.
Storage
+4 °C is recommended for lyophilized protein. For reconstituted protein in physiological buffer, short-term storage at +4 °C is recommended. For long-term storage, the protein solution should be aliquoted and then stored at -20 °C.
Shipping
At ambient temperature.
Stability
There is no decrease in performance of the reconstituted Affibody® molecule (1 mg/ml in PBS) after 10 repeated freeze and thaw cycles or after storage for 2 weeks at room temperature.
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Download Material Safety Data Sheet