Enhanced Validation of Antibodies
Validation of antibodies is an experimental confirmation that an antibody is suitable for its intended use. It is important to understand that antibody validation is application- and context-specific. Atlas Antibodies provides highly characterized antibodies further strengthened by enhanced validation, as recommended by leading researchers
To further strengthen the reliability of our antibodies we apply enhanced validation, based on the recommendations of the International Working Group for Anti-body Validation (IWGAV), as published in Nature Methods, by Uhlén M. et al (https://www.nature.com/articles/nmeth.3995).
In the article, five conceptual pillars for antibody validation are suggested to be used in an application-specific manner.
Based on these pillars, HPA and Atlas Antibodies have developed an Enhanced Validation Strategy, resulting in the following 5 methods:
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Genetic validation:
Target confirmed by siRNA knock-down.
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Orthogonal validation:
Specificity confirmed by a non-antibody-based method.
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Independent antibody validation:
Specificity is confirmed by another antibody targeting a different epitope of the protein.
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Recombinant expression validation:
The target is confirmed by an overexpressed version of the protein.
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Capture MS validation:
The presence of the target is verified by Mass Spectrometry.
At least one of the methods must be used for an antibody to receive Enhanced Validation Status in a specific application.