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Recommended Protocols

Expert Support for Your Experiments

To ensure the best results with our Triple A Polyclonals and PrecisA Monoclonals, we provide validated protocols optimized for specificity and sensitivity.

Note: Please refer to your product’s specific Data Sheet for recommended dilutions. Some optimization may be required for your unique laboratory setup.

 

 

Immunohistochemistry 
(IHC)

Standard IHC Protocol:

Best for HRP/DAB detection.

 

IHC-IF (TSA):

Optimized for Tyramide Signal Amplification.

 

IHC-IF:

Recommended IHC protocol using immunofluorescence detection.

 

IHC-IF Multiplex Protocol:

For simultaneous detection of multiple targets.

 

Ventana Discovery XT: Specific settings for automated platforms.

 

 

Western Blot (WB)

 

Standard WB Protocol: 
Our go-to method for most lysates.

 

WB BSA Blocking Protocol:
Recommended for reducing background in Western blots.

 

 

Immunocytochemistry

(ICC-IF )

Standard ICC-IF Protocol: 

Recommended ICC protocol using immunofluorescence detection.

 

Mixed Protocol & Procedures

Glycerol Removal Protocol: 
Essential step for specific downstream conjugations.

 

Antigen Blocking Protocol: 
Use this for IHC/ICC and WB validation studies.

 

Need help optimizing your protocol? 

Contact us