Immunofluorescence in Cell Lines
Subcellular localization studies are a key step in elucidating protein function and interaction. This paper introduces you to how to study subcellular localization with Triple A Polyclonals using Immunofluorescence analysis on cells.
The need for subcellular localization studies
One major rationale for investigating the subcellular location of a specific protein is that location is often tightly connected to function. For example, proteins located in the nucleus are frequently implicated in gene regulation, proteins in mitochondria with energy production, and Golgi-related proteins are often associated with protein modification and sorting.
In addition to elucidating functional characteristics of proteins, subcellular location information can also facilitate protein interaction studies. For two proteins to interact, it is a prerequisite that they be located at the same defined site. Thus, knowing the subcellular location of a protein is a key step towards understanding function and probable interaction patterns.