Enhanced Validation

Anti-TTN Antibody

titin
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human TTN
Alternative Gene Names
CMD1G, CMH9, CMPD4, FLJ32040, LGMD2J, MYLK5, TMD
Price
$437.00
Product Number
HPA007042
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
titin
Target gene
TTN
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GTVSTSCYLAVQVSEEFEKETTAVTEKFTTEEKRFVESRDVVMTDTSLTEEQAGPGEPAAPYFITKPVVQKLVEGGSVVFGCQVGGNPKPHVYWKKSGVPLTTGYRYKVSYNKQTGECKLVISMTFADDAGEYT
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000051747 (93%)

Rat ENSRNOG00000012512 (27%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Concentration
Lot dependent
Unit Size
100 µl
Concentration
Lot dependent
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
titin
Gene Name
TTN
Alternative Gene Names
CMD1G, CMH9, CMPD4, FLJ32040, LGMD2J, MYLK5, TMD
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000155657 on the Human Protein Atlas

Shin SH, Huang M, Kim SH, Choi JY

Differential Protein Expression in Congenital and Acquired Cholesteatomas

PLoS One , 2015; 10(9):e0137011. Epub 2015 Sep 3

PubMed ID: 26335306 DOI: 10.1371/journal.pone.0137011

Seki T, Yuasa S, Kusumoto D, Kunitomi A, Saito Y, Tohyama S, Yae K, Kishino Y, Okada M, Hashimoto H, Takei M, Egashira T, Kodaira M, Kuroda Y, Tanaka A, Okata S, Suzuki T, Murata M, Fujita J, Fukuda K

Generation and Characterization of Functional Cardiomyocytes Derived from Human T Cell-Derived Induced Pluripotent Stem Cells

PLoS One , 2014; 9(1):e85645. Epub 2014 Jan 21

PubMed ID: 24465630 DOI: 10.1371/journal.pone.0085645

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Alternative Antibodies
Corresponding Antigens