Enhanced Validation

Polyclonal Anti-TGFBI Antibody

transforming growth factor, beta-induced, 68kDa
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human TGFBI
Alternative Gene Names
BIGH3, CDB1, CDGG1, CSD1, CSD2, CSD3, LCD1
Open Datasheet
Price
$505.00
Product Number
HPA008612
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[7 pictures]
Immunohistochemistry analysis in human placenta and pancreas tissues using HPA008612 antibody. Corresponding TGFBI RNA-seq data are presented for the same tissues.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
transforming growth factor, beta-induced, 68kDa
Target Gene
TGFBI
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKD
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000035493 (93%)

Rat ENSRNOG00000012216 (92%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.

Validated against independent antibody Anti-TGFBI HPA017019.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA008612 on the Human Protein Atlas
Protein Name
transforming growth factor, beta-induced, 68kDa
Gene Name
TGFBI
Alternative Gene Names
BIGH3, CDB1, CDGG1, CSD1, CSD2, CSD3, LCD1
UniProt ID
Q15582
Gene (Ensembl)
ENSG00000120708
Entrez Gene ID
Entrez gene: 7045
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000120708 on the Human Protein Atlas

Zhu J, Chen X, Liao Z, He C, Hu X

TGFBI protein high expression predicts poor prognosis in colorectal cancer patients

Int J Clin Exp Pathol , 2015; 8(1):702-710. Epub 2015 Jan 1

PubMed ID: 25755764

Kuot A, Hewitt AW, Griggs K, Klebe S, Mills R, Jhanji V, Craig JE, Sharma S, Burdon KP

Association of TCF4 and CLU polymorphisms with Fuchs' endothelial dystrophy and implication of CLU and TGFBI proteins in the disease process

Eur J Hum Genet , 2012 Jun; 20(6):632-638. Epub 2012 Jan 11

PubMed ID: 22234156 DOI: 10.1038/ejhg.2011.248

Kashyap MK, Harsha H, Renuse S, Pawar H, Sahasrabuddhe NA, Kim MS, Marimuthu A, Keerthikumar S, Muthusamy B, Kandasamy K, Subbannayya Y, Prasad TS, Mahmood R, Chaerkady R, Meltzer SJ, Kumar RV, Rustgi AK, Pandey A

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Cancer Biol Ther , 2010 Oct 15; 10(8):796-810. Epub 2010 Oct 7

PubMed ID: 20686364 DOI: 10.4161/cbt.10.8.12914

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-TGFBI (HPA017019)
Corresponding Antigens
PrEST Antigen TGFBI (APrEST71267)

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