Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
High level PHGDH expression in breast is predominantly associated with keratin 5‐positive cell lineage independently of malignancy
Mol Oncol , 2015 May 14; 9(8):1636-1654. Epub 2015 May 14
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