Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Enigma proteins regulate YAP mechanotransduction
J Cell Sci , 2018 Nov 22; 131(22):jcs221788. Epub 2018 Nov 22
PDLIM5 links kidney anion exchanger 1 (kAE1) to ILK and is required for membrane targeting of kAE1
Sci Rep , 2017 Jan 3; 7:39701. Epub 2017 Jan 3
The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development.
Proteomics , 2012 Jan; 12(2):203-25. Epub 2012 Jan 4
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