Enhanced Validation

Anti-PDLIM5 Antibody

PDZ and LIM domain 5
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
Product Description
Polyclonal Antibody against Human PDLIM5
Alternative Gene Names
Enh, LIM
Price
$437.00
Product Number
HPA016740
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
PDZ and LIM domain 5
Target gene
PDLIM5
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
PFGSVSSPKVTSIPSPSSAFTPAHATTSSHASPSPVAAVTPPLFAASGLHANANLSADQSPSALSAGKTAVNVPRQPTVTSVCSETSQELAEGQRRGSQGDSKQQNGPPRKHIVERYTEFYHVPTHSDASKK
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000028273 (84%)

Rat ENSRNOG00000016419 (84%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.4 µg/ml

Immunofluorescence in cell lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 1-4 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.

Protein Name
PDZ and LIM domain 5
Gene Name
PDLIM5
Alternative Gene Names
Enh, LIM
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000163110 on the Human Protein Atlas

Elbediwy A, Vanyai H, Diaz-de-la-Loza MD, Frith D, Snijders AP, Thompson BJ

Enigma proteins regulate YAP mechanotransduction

J Cell Sci , 2018 Nov 22; 131(22):jcs221788. Epub 2018 Nov 22

PubMed ID: 30404826 DOI: 10.1242/jcs.221788

Su Y, Hiemstra TF, Yan Y, Li J, Karet HI, Rosen L, Moreno P, Frankl FE

PDLIM5 links kidney anion exchanger 1 (kAE1) to ILK and is required for membrane targeting of kAE1

Sci Rep , 2017 Jan 3; 7:39701. Epub 2017 Jan 3

PubMed ID: 28045035 DOI: 10.1038/srep39701

Li A, Ponten F, dos Remedios CG

The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development.

Proteomics , 2012 Jan; 12(2):203-25. Epub 2012 Jan 4

PubMed ID: 22253135 DOI: 10.1002/pmic.201100492

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Alternative Antibodies
Corresponding Antigens

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