Anti-NGLY1 Antibody

N-glycanase 1
Recommended Applications
Product Description
Polyclonal Antibody against Human NGLY1
Alternative Gene Names
FLJ11005, PNG1
Price
$437.00
Product Number
HPA036825
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
N-glycanase 1
Target gene
NGLY1
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEKLLETRCGR
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000006143 (87%)

Mouse ENSMUSG00000021785 (87%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Concentration
Lot dependent
Unit Size
100 µl
Concentration
Lot dependent
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Protocols
Protein Name
N-glycanase 1
Gene Name
NGLY1
Alternative Gene Names
FLJ11005, PNG1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000151092 on the Human Protein Atlas

Galeone A, Han SY, Huang C, Hosomi A, Suzuki T, Jafar-Nejad H

Tissue-specific regulation of BMP signaling by Drosophila N-glycanase 1

eLife , 2017 Aug 4; 6:e27612. Epub 2017 Aug 4

PubMed ID: 28826503 DOI: 10.7554/eLife.27612

He P, Grotzke JE, Ng BG, Gunel M, Jafar-Nejad H, Cresswell P, Enns GM, Freeze HH

A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiency in patient fibroblasts

Glycobiology , 2015 Apr 21; 25(8):836-844. Epub 2015 Apr 21

PubMed ID: 25900930 DOI: 10.1093/glycob/cwv024

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not articipate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens

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