Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-HOOK1 HPA018820.
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Hypoxia increases amyloid-β level in exosomes by enhancing the interaction between CD147 and Hook1
Am J Transl Res , 2018 Jan 15; 10(1):150-163. Epub 2018 Jan 15
Mouse Tafazzin Is Required for Male Germ Cell Meiosis and Spermatogenesis
PLoS One , 2015; 10(6):e0131066. Epub 2015 Jun 26
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