Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000021556 (46%)
Rat ENSRNOG00000018400 (45%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
MicroRNA-143 regulates cell migration and invasion by targeting GOLM1 in cervical cancer
Oncol Lett , 2018 Sep 17; 16(5):6393-6400. Epub 2018 Sep 17
The microRNA-23b/27b/24-1 cluster is a disease progression marker and tumor suppressor in prostate cancer
Oncotarget , 2014 Sep 15; 5(17):7748-7759. Epub 2014 Jul 31
The tumor-suppressive microRNA-143/145 cluster inhibits cell migration and invasion by targeting GOLM1 in prostate cancer.
J Hum Genet , 2014 Feb; 59(2):78-87. Epub 2013 Nov 28
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