Polyclonal Anti-GFAP Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000002919 (100%)
Mouse ENSMUSG00000020932 (98%)
Recommended conditions:
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-GFAP HPA063513.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000131095 on the Human Protein Atlas
Patchouli alcohol attenuates the cognitive deficits in a transgenic mouse model of Alzheimer’s disease via modulating neuropathology and gut microbiota through suppressing C/EBPβ/AEP pathway
J Neuroinflammation , 2023 Jan 30; 20:19. Epub 2023 Jan 30
Micro-RNAs Shuttled by Extracellular Vesicles Secreted from Mesenchymal Stem Cells Dampen Astrocyte Pathological Activation and Support Neuroprotection in In-Vitro Models of ALS
Cells , 2022 Dec 4; 11(23):3923. Epub 2022 Dec 4
PEG-mediated transduction of rAAV as a platform for spatially confined and efficient gene delivery
Biomater Res , 2022 Dec 2; 26:69. Epub 2022 Dec 2
Optogenetic induction of hibernation-like state with modified human Opsin4 in mice
Cell Rep Methods , 2022 Nov 14; 2(11):100336. Epub 2022 Nov 14
Fecal Microbiota Transplantation Exerts Neuroprotective Effects in a Mouse Spinal Cord Injury Model by Modulating the Microenvironment at the Lesion Site
Microbiol Spectr , 2022 Apr 25; 10(3):e00177-22. Epub 2022 Apr 25
Cold Atmospheric Plasma Does Not Affect Stellate Cells Phenotype in Pancreatic Cancer Tissue in Ovo
Int J Mol Sci , 2022 Feb 10; 23(4):1954. Epub 2022 Feb 10
Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates
Front Neurosci , 2022 Jan 11; 15:811348. Epub 2022 Jan 11
Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology
Nat Commun , 2021 Oct 7; 12:5871. Epub 2021 Oct 7
Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease
Alzheimers Dement (Amst) , 2021 Sep 14; 13(1):e12231. Epub 2021 Sep 14
Adropin correlates with aging-related neuropathology in humans and improves cognitive function in aging mice
NPJ Aging Mech Dis , 2021 Aug 30; 7:23. Epub 2021 Aug 30
High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors
J Neuroinflammation , 2021 May 16; 18:115. Epub 2021 May 16
Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer’s disease via inhibiting neuropathology and modulating gut microbiota
J Adv Res , 2021 Mar 31; 35:231-243. Epub 2021 Mar 31
Nitrosative Stress in the Frontal Cortex From Dogs With Canine Cognitive Dysfunction
Front Vet Sci , 2020 Nov 19; 7:573155. Epub 2020 Nov 19
Sulforaphene Ameliorates Neuroinflammation and Hyperphosphorylated Tau Protein via Regulating the PI3K/Akt/GSK-3β Pathway in Experimental Models of Alzheimer's Disease
Oxid Med Cell Longev , 2020 Sep 10; 2020:4754195. Epub 2020 Sep 10
Patient-Specific Retinal Organoids Recapitulate Disease Features of Late-Onset Retinitis Pigmentosa
Front Cell Dev Biol , 2020 Mar 6; 8:128. Epub 2020 Mar 6
A Toolbox of Criteria for Distinguishing Cajal–Retzius Cells from Other Neuronal Types in the Postnatal Mouse Hippocampus
eNeuro , 2020 Jan 22; 7(1):ENEURO.0516-19.2019. Epub 2020 Jan 22
EGR1 recruits TET1 to shape the brain methylome during development and upon neuronal activity
Nat Commun , 2019 Aug 29; 10:3892. Epub 2019 Aug 29
Plasma protein profiling reveals candidate biomarkers for multiple sclerosis treatment
PLoS One , 2019 May 29; 14(5):e0217208. Epub 2019 May 29
Protective effects of primary neural stem cell treatment in ischemic stroke models
Exp Ther Med , 2018 Jul 18; 16(3):2219-2228. Epub 2018 Jul 18
MicroRNA-365 alleviates morphine analgesic tolerance via the inactivation of the ERK/CREB signaling pathway by negatively targeting β-arrestin2
J Biomed Sci , 2018 Feb 7; 25:10. Epub 2018 Feb 7
Clinicoradiological characteristics, management and prognosis of primary myeloid sarcoma of the central nervous system: A report of four cases
Oncol Lett , 2017 Jul 20; 14(3):3825-3831. Epub 2017 Jul 20
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