Enhanced Validation

Polyclonal Anti-GCNA Antibody

germ cell nuclear acidic peptidase
References (3)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human GCNA
Alternative Gene Names
ACRC, NAAR1
Open Datasheet
Price
$459.00
Product Number
HPA023476
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[3 pictures]
Immunohistochemistry analysis in human testis and pancreas tissues using HPA023476 antibody. Corresponding GCNA RNA-seq data are presented for the same tissues.
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[1 pictures]
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
germ cell nuclear acidic peptidase
Target Gene
GCNA
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
QSSSDDTSGSSVARRAPKRQASCILNVQSRSGDTSGSSVARRAPKRQASSVVVIDSDSDEECHTHEEKKAKLLEINSDDESPECCHVKPAIQEPPIVISDDDNDDDNGNDLE
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000024384 (30%)

Rat ENSRNOG00000058050 (26%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA023476 on the Human Protein Atlas
Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA023476 on the Human Protein Atlas
Protein Name
germ cell nuclear acidic peptidase
Gene Name
GCNA
Alternative Gene Names
ACRC, NAAR1
UniProt ID
Q96QF7
Gene (Ensembl)
ENSG00000147174
Entrez Gene ID
Entrez gene: 93953
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000147174 on the Human Protein Atlas

Carmell MA, Dokshin GA, Skaletsky H, Hu YC, van Wolfswinkel JC, Igarashi KJ, Bellott DW, Nefedov M, Reddien PW, Enders GC, Uversky VN, Mello CC, Page DC

A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes

eLife , 2016 Oct 8; 5:e19993. Epub 2016 Oct 8

PubMed ID: 27718356 DOI: 10.7554/eLife.19993

Johnson GR, Li J, Shariff A, Rohde GK, Murphy RF

Automated Learning of Subcellular Variation among Punctate Protein Patterns and a Generative Model of Their Relation to Microtubules

PLoS Comput Biol , 1/01/01; 11(12):e1004614. Epub 2015 Dec 1

PubMed ID: 26624011 DOI: 10.1371/journal.pcbi.1004614

Did we miss your publication?

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-GCNA (HPA030870)
Corresponding Antigens
PrEST Antigen GCNA (APrEST74098)

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