Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000016866 (89%)
Mouse ENSMUSG00000008136 (89%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-FHL2 HPA005922.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Matrix mechanics controls FHL2 movement to the nucleus to activate p21 expression
Proc Natl Acad Sci U S A , 2016 Oct 14; 113(44):E6813-E6822. Epub 2016 Oct 14
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
A global view of protein expression in human cells, tissues, and organs
Mol Syst Biol , 2009; 5:337. Epub 2009 Dec 22
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