Polyclonal Anti-ESR1 Antibody
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000019768 (88%)
Rat ENSRNOG00000019358 (87%)
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-ESR1 HPA000450.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Alternative promoters and splicing create multiple functionally distinct isoforms of oestrogen receptor alpha in breast cancer and healthy tissues
Cancer Med , 2023 Sep 7; 12(18):18931-18945. Epub 2023 Sep 7
Epigenetic silencing of SALL2 confers tamoxifen resistance in breast cancer
EMBO Mol Med , 2019 Oct 28; 11(12):e10638. Epub 2019 Oct 28
Antigen Masking During Fixation and Embedding, Dissected
J Histochem Cytochem , 2016 Oct 20; 65(1):5-20. Epub 2016 Oct 20
Antibody performance in western blot applications is context-dependent.
Biotechnol J , 2014 Mar; 9(3):435-45. Epub 2014 Jan 29
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.