Polyclonal Anti-ESR1 Antibody



Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000019768 (88%)
Rat ENSRNOG00000019358 (87%)
Recommended conditions:
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-ESR1 HPA000450.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000091831 on the Human Protein Atlas
Epigenetic silencing of SALL2 confers tamoxifen resistance in breast cancer
EMBO Mol Med , 2019 Oct 28; 11(12):e10638. Epub 2019 Oct 28
Antigen Masking During Fixation and Embedding, Dissected
J Histochem Cytochem , 2016 Oct 20; 65(1):5-20. Epub 2016 Oct 20
Antibody performance in western blot applications is context-dependent.
Biotechnol J , 2014 Mar; 9(3):435-45. Epub 2014 Jan 29
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