Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Altered transcriptional regulatory proteins in glioblastoma and YBX1 as a potential regulator of tumor invasion
Sci Rep , 2019 Jul 29; 9:10986. Epub 2019 Jul 29
CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer
Dis Markers , 2018 Apr 12; 2018:3835783. Epub 2018 Apr 12
The Role of BCA2 in the Endocytic Trafficking of EGFR and Significance as a Prognostic Biomarker in Cancer
J Cancer , 2016 Dec 9; 7(15):2388-2407. Epub 2016 Dec 9
Resetting cancer stem cell regulatory nodes upon MYC inhibition
EMBO Rep , 2016 Nov 16; 17(12):1872-1889. Epub 2016 Nov 16
Microsomal membrane proteome of low grade diffuse astrocytomas: Differentially expressed proteins and candidate surveillance biomarkers
Sci Rep , 2016 Jun 1; 6:26882. Epub 2016 Jun 1
Sentinel lymph node biopsy revisited: ultrasound-guided photoacoustic detection of micrometastases using molecularly targeted plasmonic nanosensors
Cancer Res , 2014 Oct 1; 74(19):5397-5408. Epub 2014 Aug 8
Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway.
Nat Commun , 2012; 3:976
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