Enhanced Validation

Anti-CDR2L Antibody

cerebellar degeneration-related protein 2-like
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human CDR2L
Alternative Gene Names
HUMPPA
Price
$445.00
Product Number
HPA022015
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
cerebellar degeneration-related protein 2-like
Target Gene
CDR2L
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DSSWRDLRGGEEGQGEVKAGEKSLSQHVEAVDKRLEQSQPEYKALFKEIFSRIQKTKADINATKVKTHSS
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000025815 (94%)

Mouse ENSMUSG00000050910 (93%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
cerebellar degeneration-related protein 2-like
Gene Name
CDR2L
Alternative Gene Names
HUMPPA
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000109089 on the Human Protein Atlas

Schubert M, Panja D, Haugen M, Bramham CR, Vedeler CA

Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis

Acta Neuropathol , 2014 Dec; 128(6):835-852. Epub 2014 Oct 24

PubMed ID: 25341622 DOI: 10.1007/s00401-014-1351-6

Eichler TW, Totland C, Haugen M, Qvale TH, Mazengia K, Storstein A, Haukanes BI, Vedeler CA

CDR2L Antibodies: A New Player in Paraneoplastic Cerebellar Degeneration

PLoS One , 2013; 8(6):e66002. Epub 2013 Jun 18

PubMed ID: 23823982 DOI: 10.1371/journal.pone.0066002

Did we miss your publication?

Have you published using HPA022015? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

Read more

Corresponding Antigens

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch