Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000020935 (88%)
Mouse ENSMUSG00000024942 (88%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Decreased calpain 6 expression is associated with tumorigenesis and poor prognosis in HNSCC
Oncol Lett , 2017 Feb 7; 13(4):2237-2243. Epub 2017 Feb 7
Eosinophilic esophagitis–linked calpain 14 is an IL-13–induced protease that mediates esophageal epithelial barrier impairment
JCI Insight , 2016/04/01; 1(4):e86355-. Epub 2016 Apr 7
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