Enhanced Validation

Anti-ARHGAP36 Antibody

Rho GTPase activating protein 36
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human ARHGAP36
Alternative Gene Names
FLJ30058
Price
$445.00
Product Number
HPA002064
Unit Size
Concentration
Lot dependent
Availability
7 in Stock
Ships
Ready to Ship
Target Protein
Rho GTPase activating protein 36
Target Gene
ARHGAP36
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
NWDVLFQVPPHIQRQVAKRVWKSSPEALDFIRRRNLRKIQSARIKMEEDALLSDPVETSAEARAAVLAQSKPSDEGSSEEPAVPSGTARSHDDEEGAGNPPIPEQD
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000036198 (81%)

Rat ENSRNOG00000007552 (55%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
Rho GTPase activating protein 36
Gene Name
ARHGAP36
Alternative Gene Names
FLJ30058
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000147256 on the Human Protein Atlas

Nam H, Jeon S, An H, Yoo J, Lee HJ, Lee SK, Lee S

Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification

eLife , 2019 Jul 15; 8:e46683. Epub 2019 Jul 15

PubMed ID: 31305241 DOI: 10.7554/eLife.46683

Eccles RL, Czajkowski MT, Barth C, Müller PM, McShane E, Grunwald S, Beaudette P, Mecklenburg N, Volkmer R, Zühlke K, Dittmar G, Selbach M, Hammes A, Daumke O, Klussmann E, Urbé S, Rocks O

Bimodal antagonism of PKA signalling by ARHGAP36

Nat Commun , 2016 Oct 7; 7:12963. Epub 2016 Oct 7

PubMed ID: 27713425 DOI: 10.1038/ncomms12963

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Corresponding Antigens

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