Anti-ARHGAP36 Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000036198 (81%)
Rat ENSRNOG00000007552 (55%)
Recommended conditions:
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000147256 on the Human Protein Atlas
Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification
eLife , 2019 Jul 15; 8:e46683. Epub 2019 Jul 15
Bimodal antagonism of PKA signalling by ARHGAP36
Nat Commun , 2016 Oct 7; 7:12963. Epub 2016 Oct 7
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