Polyclonal Anti-ARHGAP12 Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000041225 (86%)
Rat ENSRNOG00000017791 (86%)
Recommended conditions:
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000165322 on the Human Protein Atlas
RPEL family rhoGAPs link Rac/Cdc42 GTP loading to G-actin availability
Nat Cell Biol , 2019 Jun 17; 21(7):845-855. Epub 2019 Jun 17
Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins
Nat Commun , 2015 Oct 14; 6:8623. Epub 2015 Oct 14
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