Anti-ANXA3 Antibody
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000029484 (94%)
Rat ENSRNOG00000002045 (89%)
Recommended conditions:
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Validation of protein expression in WB by comparing independent antibodies targeting different epitopes of the protein.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000138772 on the Human Protein Atlas
Differential Protein Expression Profiles of Cyst Fluid from Papillary Thyroid Carcinoma and Benign Thyroid Lesions
PLoS One , 2015; 10(5):e0126472. Epub 2015 May 15
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