Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000021497 (100%)
Mouse ENSMUSG00000019699 (100%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Investigation of molecular alterations of AKT-3 in triple-negative breast cancer.
Histopathology , 2014 Apr; 64(5):660-70. Epub 2013 Dec 12
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
Abrogation of BRAFV600E-induced senescence by PI3K pathway activation contributes to melanomagenesis
Genes Dev , 2012 May 15; 26(10):1055-1069
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