Polyclonal Anti-AHCY Antibody
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000048087 (96%)
Rat ENSRNOG00000017777 (96%)
Recommended conditions:
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 0.04-0.4 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
All characterization data for ENSG00000101444 on the Human Protein Atlas
RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins.
J Proteome Res , 2013 Jan 4; 12(1):299-307. Epub 2012 Dec 20
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