Enhanced Validation

Monoclonal Anti-TMEM119 Antibody

transmembrane protein 119
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human TMEM119
Open Datasheet
Price
$505.00
Product Number
AMAb91528
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[5 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb91528 antibody. Corresponding TMEM119 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in human cell line SH-SY5Y.
Show all images
[1 pictures]
Immunofluorescence staining of SH-SY5Y cells using the Anti-TMEM119 monoclonal antibody, showing specific staining to the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
transmembrane protein 119
Target Gene
TMEM119
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000054675 (52%)

Rat ENSRNOG00000000700 (46%)

Clonality
Monoclonal
Clone ID
CL8714
Isotype
IgG1
Host
MOUSE
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91528 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91528 on the Human Protein Atlas
Protein Name
transmembrane protein 119
Gene Name
TMEM119
UniProt ID
Q4V9L6
Gene (Ensembl)
ENSG00000183160
Entrez Gene ID
Entrez gene: 338773
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Pandit K, Petrescu J, Cuevas M, Stephenson W, Smibert P, Phatnani H, Maniatis S

An open source toolkit for repurposing Illumina sequencing systems as versatile fluidics and imaging platforms

Sci Rep , 2022 Mar 24; 12:5081. Epub 2022 Mar 24

PubMed ID: 35332182 DOI: 10.1038/s41598-022-08740-w

Did we miss your publication?

Have you published using AMAb91528? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-TMEM119 (HPA051870)
Anti-TMEM119 (HPA052650)
Corresponding Antigens
PrEST Antigen TMEM119 (APrEST85802)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch