Enhanced Validation

Monoclonal Anti-STAT3 Antibody

signal transducer and activator of transcription 3 (acute-phase response factor)
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human STAT3
Alternative Gene Names
APRF
Open Datasheet
Price
$505.00
Product Number
AMAb90777
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human endometrium and pancreas tissues using AMAb90777 antibody. Corresponding STAT3 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STAT3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[1 pictures]
Immunofluorescence staining of A549 cells using the Anti-STAT3 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
signal transducer and activator of transcription 3 (acute-phase response factor)
Target Gene
STAT3
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000004040 (99%)

Rat ENSRNOG00000019742 (99%)

Clonality
Monoclonal
Clone ID
CL0492
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90777 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90777 on the Human Protein Atlas
Protein Name
signal transducer and activator of transcription 3 (acute-phase response factor)
Gene Name
STAT3
Alternative Gene Names
APRF
UniProt ID
P40763
Gene (Ensembl)
ENSG00000168610
Entrez Gene ID
Entrez gene: 6774
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Lu H, Wu S, Chen H, Huang Y, Qiu G, Liu L, Li Y

Crizotinib induces apoptosis of lung cancer cells through JAK-STAT pathway

Oncol Lett , 2018 Sep 4; 16(5):5992-5996. Epub 2018 Sep 4

PubMed ID: 30333870 DOI: 10.3892/ol.2018.9387

Zhou T, Lin H, Cheng Z, Ji C, Zhang C, Tian J

Mechanism of microRNA-146a-mediated IL-6/STAT3 signaling in lumbar intervertebral disc degeneration

Exp Ther Med , 2017 Jun 15; 14(2):1131-1135. Epub 2017 Jun 15

PubMed ID: 28810568 DOI: 10.3892/etm.2017.4611

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-STAT3 (AMAb90776)
Anti-STAT3 (HPA058603)
Corresponding Antigens
PrEST Antigen STAT3 (APrEST77483)

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